一、光學顯微鏡的分類方法
光(guang)(guang)學(xue)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)有(you)多種(zhong)分(fen)類(lei)方法,按(an)(an)使(shi)用(yong)目(mu)鏡(jing)(jing)(jing)(jing)的(de)數目(mu)可分(fen)為(wei)(wei)三目(mu),雙(shuang)目(mu)和(he)(he)(he)單(dan)目(mu)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing);按(an)(an)圖(tu)像(xiang)(xiang)是否(fou)有(you)立(li)體(ti)感可分(fen)為(wei)(wei)立(li)體(ti)視(shi)覺和(he)(he)(he)非立(li)體(ti)視(shi)覺顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing);按(an)(an)觀察(cha)對像(xiang)(xiang)可分(fen)為(wei)(wei)生物和(he)(he)(he)金相(xiang)(xiang)(xiang)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng);按(an)(an)光(guang)(guang)學(xue)原理可分(fen)為(wei)(wei)偏光(guang)(guang),相(xiang)(xiang)(xiang)襯和(he)(he)(he)微(wei)(wei)(wei)(wei)(wei)分(fen)干涉對比顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng);按(an)(an)光(guang)(guang)源類(lei)型(xing)可分(fen)為(wei)(wei)普(pu)通光(guang)(guang)、熒(ying)光(guang)(guang)、紅外光(guang)(guang)和(he)(he)(he)激光(guang)(guang)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng);按(an)(an)接收器類(lei)型(xing)可分(fen)為(wei)(wei)目(mu)視(shi)、攝影和(he)(he)(he)電視(shi)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng)。常用(yong)的(de)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)有(you)雙(shuang)目(mu)連(lian)續(xu)變倍體(ti)視(shi)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)、金相(xiang)(xiang)(xiang)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)、偏光(guang)(guang)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)、紫外熒(ying)光(guang)(guang)顯(xian)(xian)(xian)(xian)微(wei)(wei)(wei)(wei)(wei)鏡(jing)(jing)(jing)(jing)等(deng)。
二、光學顯微鏡的使用規程
1、實驗時要把顯微鏡放(fang)在(zai)桌(zhuo)面上稍偏左(zuo)的(de)位置,鏡座應距桌(zhuo)沿6~7 cm左(zuo)右。
2、打開(kai)光(guang)源開(kai)關,調節光(guang)強到合適大小。
3、轉(zhuan)動物(wu)鏡(jing)轉(zhuan)換器,使(shi)低倍(bei)鏡(jing)頭(tou)正對載物(wu)臺上的(de)通光孔。先把(ba)鏡(jing)頭(tou)調(diao)節至距載物(wu)臺1~2cm左右處,然后用左眼(yan)注視目鏡(jing)內,接著調(diao)節聚(ju)光器的(de)高度,把(ba)孔徑光闌調(diao)至最大,使(shi)光線通過聚(ju)光器射入到鏡(jing)筒內,這時視野(ye)內呈明亮(liang)的(de)狀態(tai)。
4、將所要觀(guan)察的(de)玻片(pian)(pian)放在載(zai)物臺上,使玻片(pian)(pian)中被觀(guan)察的(de)部分位于(yu)通光孔的(de)正中央,然后用標本夾夾好載(zai)玻片(pian)(pian)。
5、先用(yong)低倍(bei)鏡(jing)(jing)觀察(cha)(cha)(物鏡(jing)(jing)10X、目鏡(jing)(jing)10x)。觀察(cha)(cha)之前,先轉(zhuan)動(dong)(dong)(dong)粗動(dong)(dong)(dong)調(diao)(diao)焦手輪,使載(zai)物臺上升,物鏡(jing)(jing)逐漸接近玻片。需要注意,不(bu)能使物鏡(jing)(jing)觸及玻片,以(yi)防鏡(jing)(jing)頭將玻片壓碎。然后,左眼注視目鏡(jing)(jing)內(nei),同(tong)時右眼不(bu)要閉合(要養成睜(zheng)開雙眼用(yong)顯微鏡(jing)(jing)進行觀察(cha)(cha)的(de)習慣(guan),以(yi)便在觀察(cha)(cha)的(de)同(tong)時能用(yong)右眼看(kan)著(zhu)繪圖),并轉(zhuan)動(dong)(dong)(dong)粗動(dong)(dong)(dong)調(diao)(diao)焦手輪,使載(zai)物臺慢慢下(xia)降,不(bu)久即可看(kan)到玻片中材料的(de)放大物像。
6、如果在視野(ye)內(nei)看(kan)到的物(wu)像不(bu)符合實(shi)驗要(yao)求(物(wu)像偏離視野(ye)),可慢(man)慢(man)調節(jie)載物(wu)臺移(yi)動手(shou)柄。調節(jie)時應注意,玻(bo)片(pian)移(yi)動的方向(xiang)與視野(ye)中看(kan)到的物(wu)像移(yi)動的方向(xiang),正好(hao)相反。如果物(wu)像不(bu)甚清(qing)晰(xi),可以調節(jie)微(wei)動調焦手(shou)輪,直至物(wu)像清(qing)晰(xi)為(wei)止。
7、一(yi)般具有正(zheng)常功(gong)能的顯(xian)微(wei)(wei)鏡(jing),低(di)倍物(wu)鏡(jing)和高(gao)倍物(wu)鏡(jing)基本齊(qi)焦,在用低(di)倍物(wu)鏡(jing)觀(guan)察清晰(xi)時,換高(gao)倍物(wu)鏡(jing)應可以見到物(wu)像,但物(wu)像不一(yi)定(ding)很清晰(xi),可以轉動微(wei)(wei)動調(diao)焦手輪進行(xing)調(diao)節。
8、在轉換高(gao)倍物(wu)鏡(jing)(jing)并且(qie)看清物(wu)像之(zhi)后,可(ke)以根據需(xu)要調節孔徑光闌的大小(xiao)或聚光器的高(gao)低,使光線符合(he)要求(qiu)(一般將低倍物(wu)鏡(jing)(jing)換成高(gao)倍物(wu)鏡(jing)(jing)觀察(cha)時(shi),視(shi)野要稍變暗一些,所以需(xu)要調節光線強弱)。
9、觀察(cha)完畢(bi),應(ying)先將(jiang)(jiang)物(wu)鏡鏡頭從通光孔處移開,然后將(jiang)(jiang)孔徑光闌(lan)調至最(zui)大,再(zai)將(jiang)(jiang)載物(wu)臺緩緩落下(xia),并(bing)檢(jian)查零件有無(wu)損傷(特(te)別要注(zhu)意(yi)檢(jian)查物(wu)鏡是否沾水沾油(you),如(ru)沾了水或油(you)要用鏡頭紙擦凈),檢(jian)查處理完畢(bi)后即可裝箱。